Human GSAP/PION ELISA Kit

Designed for quantitative measurement of Gamma-Secretase Activating Protein (GSAP, also known as PION) in human samples, this Sandwich ELISA from Assay Genie delivers high sensitivity and specificity for research into gamma-secretase regulation and amyloid beta peptide generation linked to Alzheimer's disease pathogenesis. The double-antibody format is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Key specifications include a sensitivity of 0.938 ng/mL and a detection range of 1.563-100 ng/mL. The kit is stored at 2-8°C for 12 months and uses a colorimetric detection method, with absorbance read at 450 nm following addition of TMB substrate and stop solution.

Linearity is documented across 1:2 to 1:8 dilutions, spanning 82-105% for serum, 82-100% for EDTA plasma, and 80-101% for heparin plasma. Recovery performance is reported as 85-103% (average 96%) for serum, 91-104% (average 97%) for EDTA plasma, and 90-99% (average 95%) for heparin plasma.