Human GRK5 ELISA Kit (HUFI07830)

Quantitative immunoassay work measuring endogenous G protein-coupled receptor kinase 5 (GRK5) in human matrices is supported by a research-use-only reagent from Assay Genie for cardiovascular biology, neuroscience signaling, and oncology applications. The Human GRK5 ELISA plate configuration supports 96 determinations per run using a colorimetric absorbance readout. GRK5 phosphorylates activated GPCRs and drives β-arrestin-mediated receptor desensitization, and the kinase also translocates to the nucleus where it phosphorylates histone deacetylase 5 (HDAC5), influencing transcriptional programs that underlie cardiac hypertrophy, maladaptive remodeling after pressure overload, and progression toward heart failure. Separate research connects GRK5 activity to β-adrenergic signaling in hypertension and to regulation of opioid and dopamine receptors in neuroscience contexts. The same kinase has been documented to accumulate in the nuclei of breast and prostate tumor cells, where elevated levels correlate with proliferation and prognostic markers. The kit accepts human serum or plasma specimens and is configured for screening projects and translational workflows. Researchers should refer to the Certificate of Analysis enclosed with each lot for current working ranges, lot-specific calibrators, and validated cross-reactivity data. Long-term storage should follow the printed protocol; aliquot the standard after reconstitution to limit freeze-thaw cycles, and protect the substrate from light until the final incubation step.