Human GRIPAP1 ELISA Kit (HUFI04032)

GRIPAP1 (GRIP1-associated protein 1), also designated GRASP-1, operates as a neuronal scaffolding factor that governs AMPA receptor trafficking at glutamatergic synapses and influences synaptic plasticity. This Assay Genie kit supports quantitative measurement of human GRIPAP1 for neuroscience and cell biology research, including investigations into excitatory neurotransmission, receptor trafficking mechanisms, and neurological disease models.

The assay employs a microplate-based immunoassay workflow with sufficient reagents for 96 individual determinations. Each kit includes a pre-coated microplate, capture antibody, standards, and substrate buffer necessary for the procedure.

Recommended sample input is 100 uL per well, with a total assay runtime of approximately 3.5 hours at room temperature (18-25°C). Reagents should be stored refrigerated at 4°C; reconstituted components require aliquoting to minimize freeze-thaw cycles and preserve antibody activity for the shelf life of the kit.

Applications include in vitro studies of neuronal cell cultures, quantification in tissue homogenates, and translational research into synaptic dysfunction. The kit is designated for research use only.