Human Grb2 (Growth Factor Receptor Bound Protein 2) ELISA Kit (HUES02873)

This Sandwich ELISA from Assay Genie quantitatively measures human Grb2 in serum, plasma, and other biological fluids using a colorimetric detection method on a 96-well microplate. Grb2 is a cytoplasmic adaptor protein containing a central SH2 domain flanked by two SH3 domains, linking activated receptor tyrosine kinases to the Ras-MAPK cascade and influencing downstream pathways involved in cell proliferation, migration, and differentiation. Investigators use this assay to characterize growth factor receptor signaling, evaluate adaptor protein expression in tumor samples and cancer cell lines, and profile phosphorylation-dependent signaling networks in oncology and signal transduction research. The procedure provides reproducible quantification across a defined linear range with low cross-reactivity to related SH2- and SH3-containing proteins. For best results, store unopened components refrigerated at 2-8°C, bring the microplate and buffers to room temperature before initiating the assay, and limit freeze-thaw cycles of the lyophilized standards and concentrated detection antibody to preserve binding activity.