Human Granzyme H (GZMH) ELISA Kit

Granzyme H is a chymotrypsin-family serine protease stored in the cytolytic granules of cytotoxic T lymphocytes and natural killer cells, where it contributes to targeted cell death. The Assay Genie ELISA kit quantifies the human isoform of this enzyme in research samples using matched antibody pairs immobilized on a microplate, with a chromogenic enzymatic step that produces an absorbance change proportional to GZMH concentration in each well. Each kit is supplied as a complete reagent set, including pre-coated plate, lyophilized and liquid standards, detection antibody, wash buffer, dilution buffer, and substrate solution, sufficient for ninety-six individual determinations per plate. Researchers working on immune cytotoxicity, antiviral responses, tumor immunology, and granule-mediated apoptosis apply this kit on clarified cell lysates, tissue homogenates, and body-fluid fractions prepared from human specimens. The protocol follows conventional incubation and washing steps run at room temperature, with absorbance read on a standard plate reader; results are interpolated from a standard curve across the working assay range. Storage of unopened kit components under refrigerated conditions preserves reagent activity through the stated shelf life, and repeated freeze-thaw of reconstituted standards should be avoided by aliquoting on first use. Research use only.