Human GRAMD1B ELISA Kit (HUFI04042)

The GRAMD1B gene encodes a multi-pass transmembrane protein localized to the endoplasmic reticulum, where it participates in non-vesicular cholesterol transport between organelle membranes and contains a GRAM domain implicated in lipid binding. Researchers employ this Assay Genie kit to quantify GRAMD1B levels in human biospecimens, supporting investigations into lipid homeostasis, cholesterol trafficking, and membrane contact site biology. Each kit contains a sealed microplate pre-coated with capture antibody, lyophilized recombinant standards, biotinylated detection reagent, streptavidin-HRP conjugate, and assay buffer. The recommended sample volume is 100 uL per well, with the enzymatic reaction stopped by addition of stop solution before absorbance measurement on a standard plate reader. Reconstituted standards and concentrated reagents should be aliquoted and stored at -20°C; unopened kit components should be refrigerated at 4°C and used within the stated shelf life. Results are interpolated from the included standard curve and reported in ng/mL. For research use only; not intended for diagnostic procedures.