Human GPR177 ELISA Kit (HUFI07847)

Wnt signaling research increasingly requires quantitative readouts of GPR177, the conserved transmembrane chaperone responsible for trafficking Wnt ligands from the Golgi apparatus to the plasma membrane. This ELISA Kit from Assay Genie provides a complete colorimetric assay configured for human specimen analysis, with components sufficient for 96 determinations.

GPR177 (also known as Wntless, WLS, or EVI) cycles between the endoplasmic reticulum, Golgi, and cell surface, escorting lipid-modified Wnt proteins through the secretory pathway. Researchers studying colorectal cancer, hepatocellular carcinoma, triple-negative breast cancer, and pancreatic ductal adenocarcinoma use GPR177 quantification as an upstream indicator of Wnt ligand bioavailability. Additional applications include investigations of osteoblast differentiation, intestinal stem cell renewal, and neurodevelopmental patterning.

The kit includes a pre-coated microplate, matched capture and detection antibodies, recombinant human reference standards, dilution buffers, concentrated wash buffer, TMB substrate, and stop solution. Reconstituted reagents should be stored refrigerated to preserve activity, and the sealed kit is shipped under refrigerated conditions. Researchers are advised to follow the enclosed protocol for sample preparation, incubation times, and standard curve generation. The microplate format supports standard ELISA workflow and generates results suitable for downstream pathway analysis. For research use only.