Human GPR142 ELISA Kit (HUFI04057)

This Assay Genie kit enables quantitative measurement of GPR142, an orphan G protein-coupled receptor implicated in glucose-stimulated insulin secretion from pancreatic beta cells, with a standard curve range of 0.156–10 ng/mL and a minimum detectable dose of approximately 0.05 ng/mL. The assay is supplied as a complete sandwich ELISA kit with a pre-coated 96-well microplate, capture antibody, biotinylated detection antibody, recombinant GPR142 standards, streptavidin-HRP conjugate, substrate solution, and wash buffer sufficient for 96 determinations performed in vitro. Investigators studying metabolic disease pathways, including type 2 diabetes and obesity, can apply this kit to serum, plasma, and cell culture supernatant specimens using 100 µL of sample per well to monitor circulating GPR142 levels. All components should be stored at 4°C until first use, with working standards prepared fresh and any unused reagents aliquoted and frozen to limit freeze-thaw cycles. Intended for research use only, the assay supports studies exploring GPR142 as a candidate therapeutic target for glycemic control in human and rodent model systems.