Human GPIHBP1 (Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1) ELISA KIT (HUFI05164)

GPIHBP1 is a glycosylphosphatidylinositol-anchored protein expressed on capillary endothelial cells, where it binds lipoprotein lipase and shuttles it to the vascular lumen. This binding function is essential for the hydrolysis of triglycerides carried in chylomicrons and very-low-density lipoproteins, making GPIHBP1 a focal molecule in lipid metabolism research, atherosclerosis studies, and investigations of familial chylomicronemia syndrome.

The Assay Genie quantification kit measures circulating GPIHBP1 levels in human samples specifically, supporting studies on hypertriglyceridemia, metabolic syndrome, and cardiovascular disease biomarkers. Researchers use this assay to assess GPIHBP1 expression in patient cohorts, examine correlations with triglyceride concentrations, and explore mechanisms underlying lipoprotein lipase delivery to the capillary surface.

Unopened kit components should be stored at 4 degrees C; reconstituted standards and the biotinylated detection antibody should be kept frozen at -20 degrees C in single-use aliquots to limit freeze-thaw cycles. Allow all reagents to reach room temperature before initiating the protocol, and use 50 microliters of sample per well when diluting into the assay buffer. The kit ships in a sealed foil pouch with desiccant to protect the pre-coated microplate from moisture.