Human GPAA1 ELISA Kit (HUFI04069)

Glycosylphosphatidylinositol anchor attachment 1 (GPAA1) is the catalytic subunit of the GPI transamidase complex, the multi-subunit machinery that tethers pre-assembled glycosylphosphatidylinositol anchors to nascent client proteins in the endoplasmic reticulum membrane. The Assay Genie ELISA Kit for human GPAA1 enables quantitative measurement of this GPI transamidase subunit in research specimens, supporting investigations of anchor biosynthesis, cancer surface proteome remodeling, hematopoietic differentiation, and inherited glycosylation disorders such as Hyperphosphatasia with Mental Retardation Syndrome 2 (Mabry syndrome).

Supplied as a complete 96 Assays pack from Assay Genie, the kit includes pre-coated microplate wells, a matched capture and detection antibody pair, recombinant GPAA1 calibrator, concentrated wash buffer, diluents, chromogenic substrate, and stop solution. Researchers studying GPI anchor biology, oncology biomarker discovery, or the molecular basis of congenital GPI deficiency use the kit to measure GPAA1 concentrations in matched human serum, EDTA plasma, and cell or tissue lysate samples across case and control cohorts.

For research use only. Refrigerate the sealed kit; allow plates and reagents to equilibrate to room temperature before each run, and protect the chromogenic substrate from prolonged light exposure. Reconstituted standards should be aliquoted and stored frozen to limit repeated freeze-thaw cycles.