Human GP4/CD36 ELISA Kit

A high-quality sandwich ELISA kit from Assay Genie designed for the precise quantitative measurement of human GP4 (CD36) levels in research samples. It uses a colorimetric, double-antibody sandwich format and is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. GP4 (CD36) is involved in lipid metabolism, inflammation, and immune responses, and its dysregulation has been linked to atherosclerosis, diabetes, and thrombosis, making it a useful biomarker for studying these conditions. Reagents are stored at 2–8°C for up to 12 months. Recovery in serum averages 95%, in EDTA plasma 96%, and in heparin plasma 94%, while linearity across 1:2 to 1:8 dilutions falls within 80–105% across the tested matrices. The standard protocol involves reagent equilibration, a 90-minute primary incubation at 37°C, a 60-minute biotin-labelled detection antibody incubation, a 30-minute HRP-Streptavidin binding step, TMB colour development for 10–20 minutes in the dark, and absorbance reading at 450 nm after adding stop solution.