Human GP repeat ELISA Kit (HUFI04067)

The Assay Genie Human GP repeat ELISA Kit provides researchers with a quantitative immunoassay platform validated specifically for measuring GP repeat protein levels in human-derived research specimens, including serum, plasma, and cell culture supernatant samples. Each kit contains pre-coated plate wells, capture antibodies, detection reagents, lyophilized standards, and dilution buffers required to perform the full assay protocol in a single plate run covering ninety-six determinations using 100 μL of sample per well.

The workflow involves incubation with biotinylated detection antibody followed by streptavidin-HRP conjugate. After washing and addition of substrate, color development is measured with a standard microplate reader. Researchers should bring all reagents to room temperature before use and avoid repeated freeze-thaw cycles of the standard and conjugate vials to preserve activity. Unopened kit components are stored at 4 degrees C until first use, with the reconstituted standard aliquoted and frozen for subsequent runs.

Typical run time is approximately 3-4 hours from sample addition to final read. This kit is intended for research use only and is not approved for diagnostic or clinical decision-making. Performance in matrices such as tissue homogenates has not been validated by the manufacturer and would require independent verification by the end user.