Human GOPC ELISA Kit (HUFI07858)

For researchers needing quantitative measurement of human Golgi-associated PDZ and coiled-coil motif-containing (GOPC) protein, this immunoassay from Assay Genie offers a standardized workflow. GOPC, also designated PIST or CAL, operates as a scaffold that directs membrane cargo between the trans-Golgi network and endosomes, with documented roles in CFTR trafficking regulation, tight junction maintenance, and glioblastoma cell biology.

Investigators studying epithelial polarization, receptor sorting, or vesicle trafficking pair this ELISA with orthogonal methods such as western blot and PCR to confirm changes in endogenous GOPC expression. The kit has been applied to cell lysates and tissue homogenates prepared from human-derived samples, supporting diverse membrane-trafficking research programs.

Each kit ships with all necessary buffers, detection reagents, and a recombinant human GOPC calibrator for standard curve generation, sufficient for 96 Assays. Reconstituted components should be aliquoted to limit freeze-thaw exposure, and the unopened kit remains stable when refrigerated.

Quality control data from the manufacturer cover intra-assay and inter-assay precision profiling, with each lot validated before release. Intended for research use only, this reagent is not cleared for diagnostic procedures performed on patient specimens.