Human Gonadotropin Releasing Hormone (GnRH) ELISA Kit

The Assay Genie Human Gonadotropin Releasing Hormone (GnRH) ELISA Kit is a quantitative sandwich immunoassay pre-coated onto a microplate strip format, calibrated for measuring endogenous GnRH in human serum, plasma, tissue homogenates, cell lysates, and cell culture supernatants. GnRH is a ten-amino-acid hypothalamic decapeptide that pulses to drive the pituitary-gonadal axis, so quantitative measurement of this neuropeptide supports research in reproductive endocrinology, infertility, hypogonadism, pubertal disorders, and the evaluation of GnRH analog therapies.

The working detection range is 1.23 to 100 pg/mL, with sensitivity around 0.5 pg/mL. The standard protocol uses 50 uL of sample or standard per well, with a biotinylated detection antibody, streptavidin-HRP, and chromogenic readout at 450 nm; assay completion typically takes under three hours. Unopened kits should be refrigerated; reconstituted standards and concentrated detection reagents are best aliquoted and stored frozen at -20 degrees C, avoiding repeated freeze-thaw cycles of the standard curve. For research use only.