Human GNPNAT1 ELISA Kit (HUFI04073)

GNPNAT1 (Glucosamine-Phosphate N-Acetyltransferase 1) is the rate-limiting enzyme of the hexosamine biosynthetic pathway, catalyzing the acetylation of glucosamine-6-phosphate to N-acetylglucosamine-6-phosphate, a precursor for UDP-GlcNAc and protein O-GlcNAcylation. This Assay Genie ELISA kit delivers a quantitative immunoassay for measuring GNPNAT1 in human research samples, supporting investigations into cancer metabolism, glycosylation biology, and glucose homeostasis.

Published studies of the hexosamine biosynthetic pathway have linked elevated GNPNAT1 activity to metabolic reprogramming in multiple tumor types, positioning the protein as a candidate biomarker in oncology and metabolic disease research. Distributed as a 96 Assays kit, the assay accommodates high-throughput screening of experimental or archived clinical specimens.

The colorimetric workflow is straightforward: bring reagents to room temperature before use, run the protocol as described in the product insert, and capture absorbance on a standard microplate reader. Component storage conditions are detailed in the packaging; protect light-sensitive reagents from exposure during the procedure. The kit is intended for research use only.