Human GNGT1 ELISA Kit (HUFI04085)

Quantitative measurement of GNGT1 (G Protein Subunit Gamma Transducin 1), a heterotrimeric G-protein gamma subunit essential for the phototransduction cascade in retinal photoreceptors. The target protein has a molecular weight of approximately 8.5 kDa and associates with the GNAT1 alpha and GNB1 beta subunits to mediate light-induced signal amplification in rod outer segments. This Assay Genie immunoassay supports in vitro studies on G-protein coupled receptor signaling, retinal biology, and downstream effector pathways relevant to inherited retinal dystrophies and other vision research applications.

The kit includes a pre-coated microplate, capture antibody, detection reagent, recombinant standards, buffers, and substrate supplied in individual vials. Keep all reagents refrigerated between runs and protect the substrate vial from light to preserve enzymatic activity. The full workflow is generally completed within a single working day, with absorbance read on a standard plate reader.