Human GMPPA ELISA Kit (HUFI04086)

GDP-mannose pyrophosphorylase A (GMPPA) is the catalytic enzyme that converts GTP and mannose-1-phosphate into GDP-mannose, a nucleotide-sugar donor essential for protein N-glycosylation and O-mannosylation in the human endoplasmic reticulum. The Human GMPPA ELISA Kit from Assay Genie provides a quantitative immunoassay for measuring this enzyme in research samples, supporting studies of congenital disorders of glycosylation and the alacrima-achalasia-additional neurologic findings syndrome linked to GMPPA mutations.

Beyond inherited metabolic disease research, the kit is used in cancer glycobiology where altered GDP-mannose availability reshapes tumor-cell surface glycans and modulates immune recognition. The microplate configuration supports simultaneous standard-curve and unknown-sample wells within the same analytical run. Reagents ship on cold packs; store refrigerated upon arrival and protect conjugate vials from prolonged light exposure to preserve activity.

Each kit includes a pre-coated plate, detection antibody, recombinant standards, wash buffer, substrate, and stop solution sufficient for one full plate of determinations. The assay is for research use only and has not been validated for diagnostic decision-making. Best results are obtained by running all unknowns in duplicate and processing a freshly prepared standard curve on every plate to enable reliable interpolation of concentration values.