Human Glypican 3 (GPC3) ELISA Kit

Quantify Glypican-3 (GPC3), a glycophosphatidylinositol-anchored heparan sulfate proteoglycan implicated in hepatocellular carcinoma, with this immunoassay kit from Assay Genie. GPC3 is minimally expressed in healthy adult liver but is upregulated in HCC tumor tissue, making it a widely studied serological and tissue-level biomarker for liver cancer research, oncofetal antigen profiling, and preclinical evaluation of GPC3-directed therapeutics such as bispecific antibodies, antibody-drug conjugates, and chimeric antigen receptor T-cell constructs.

This in vitro research-use-only kit supplies sufficient reagents for ninety-six individual determinations and is validated with human serum samples, heparin and EDTA plasma-derived specimens, and tissue homogenate preparations. The workflow uses a matched-pair antibody configuration on a pre-coated microplate, with detection achieved through an enzyme-mediated substrate reaction that yields a measurable absorbance change. The protocol adapts readily to biomarker discovery studies, longitudinal patient sample profiling, validation of cell-line and xenograft GPC3 expression, and screening of antibody-based candidates during early-stage development. All reagents are supplied in clearly labeled vials, and the kit ships with a microplate, calibrated standard, detection antibody working solution, wash buffer, substrate, and stop solution, accompanied by lot information to support reproducibility across runs.