Human Glypican-1 (GPC1) ELISA Kit

Quantify circulating Glypican-1 with this in vitro assay from Assay Genie, providing sensitive measurement of the heparan sulfate proteoglycan in human samples. Glypican-1 (GPC1) is a GPI-anchored cell surface proteoglycan that has attracted attention as a candidate biomarker in pancreatic cancer, where exosomal and soluble GPC1 levels in peripheral blood distinguish malignant from benign pancreatic disease. This ELISA-format immunoassay uses a pre-coated microplate with anti-GPC1 capture antibody and produces a measurable signal proportional to GPC1 concentration. The kit includes a recombinant human GPC1 standard curve for quantification, intended for research use only. Standard protocol runs approximately 3-4 hours from sample addition to final absorbance read, with sample volume of 100 uL per well. Store kit components refrigerated between uses, and avoid repeated freeze-thaw cycles of reconstituted standards and detection antibody working solutions. Each kit supplies microplate strips, lyophilized standard vial, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer bottle, and substrate solution for one full plate run, with break-apart strip wells allowing smaller batch sizes. Researchers investigating tumor-derived extracellular vesicles, hepatocellular carcinoma biomarkers, or GPC1 shedding mechanisms will find this assay compatible with standard plate-reader workflows and downstream data analysis.