Human Glycosylated Serum Protein ELISA Kit

A sandwich ELISA in double antibody format from Assay Genie, developed for the precise detection of glycosylated serum proteins in human samples. Colorimetric detection supports accurate and reliable results across research applications. Glycosylated serum proteins are involved in cellular communication, immune response, and disease development, and monitoring them can offer insights into the progression of conditions such as diabetes, cancer, and inflammation.

The kit is reactive with human samples and validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Sensitivity is 46.875 nmol/mL with a working range of 78.125–5000 nmol/mL. Storage is at 2-8°C for 12 months, with coated strips and lyophilized standards also suitable for storage at -20°C for 12 months. The workflow uses a biotin-labelled detection antibody, HRP-Streptavidin conjugate (SABC), and TMB substrate, with absorbance measured at 450 nm. Linearity spans 81–102% across serum, EDTA plasma, and heparin plasma at serial dilutions, and recovery averages 95–97% across the same matrices.