Human Glycosylated HbA1C ELISA Kit

Designed for the accurate measurement of glycosylated hemoglobin A1c levels in human serum and plasma samples, this immunoassay kit from Assay Genie supports in vitro quantitative determination of Human HbA1c concentrations across serum, plasma, and other biological fluids. Glycosylated hemoglobin A1c serves as a valuable biomarker for long-term glucose monitoring in diabetic patients, reflecting average blood glucose levels over a 2–3 month period, making the assay useful for managing diabetes and assessing the effectiveness of treatment plans.

The assay uses a Competitive ELISA format coated with antibody, offering a sensitivity of 37.5 pg/ml and a working range of 62.5–4000 pg/ml. Precision is characterised by intra-assay CV below 8% and inter-assay CV below 10%. Validated recovery rates in serum, EDTA plasma, and UFH plasma fall between 81% and 105%, and linearity has been demonstrated across serial dilutions in the same matrices, with high specificity and reproducibility reported.

Components supplied include a dismountable microplate, lyophilised standard, dilution buffers, biotin-labelled antibody, HRP-Streptavidin conjugate, TMB substrate, stop solution, wash buffer, and plate sealers, with most reagents stored at 4°C for 6 months. Sample preparation guidance covers serum, plasma, urine, cerebrospinal fluid, cell culture supernatant, cell lysates, and tissue homogenates. The kit is intended for Research Use Only.