Human Glycogen Phosphorylase, Liver (PYGL) ELISA Kit

For laboratories investigating the rate-limiting enzyme of hepatic glycogenolysis, this Assay Genie ELISA kit provides quantitative measurement of liver glycogen phosphorylase (PYGL) protein in human research samples. The PYGL isoform governs the release of glucose-1-phosphate from stored hepatic glycogen, and altered expression or activity is implicated in insulin resistance, fasting hyperglycemia, type 2 diabetes, and inherited glycogen storage diseases such as Hers disease.

The procedure accepts tissue homogenates as its validated sample matrix, supporting experiments that prepare liver biopsy material, perfused-liver lysates, or hepatocyte cell culture extracts through standard homogenization protocols. With reactivity directed at the human target, the kit fits clinical sample cohorts, xenograft tissue studies, and recombinant expression work using human PYGL constructs.

Kitted components include a pre-coated plate and calibration materials sufficient for 96 individual determinations, supplying enough throughput for mid-sized screening campaigns. The workflow is built around a standard microplate reader and is intended for research use only, complementing existing absorbance-based workflows in metabolism, endocrinology, and hepatic biology laboratories.