Human Glutathione S Transferase Alpha 1 (GSTa1) ELISA Kit

Quantitative measurement of human Glutathione S-Transferase Alpha 1 supports research into phase II detoxification pathways, hepatic injury mechanisms, and tumor biology. This cytosolic protein catalyses conjugation of reduced glutathione to electrophilic substrates, contributing to xenobiotic clearance and cellular defense against oxidative stress. Researchers studying drug-induced liver injury, glutathione-mediated detoxification, and chemoresistance rely on accurate quantitation of circulating and intracellular GSTa1 levels to track enzyme expression across experimental conditions.

This colorimetric ELISA from Assay Genie is configured for human reactive specimens and accommodates cell lysates, tissue homogenates, and cell culture supernatants generated from in vitro experiments. The assay format supports investigation into mechanisms where elevated GSTa1 has been documented as a contributor to platinum-based drug efflux and treatment response variation. Additional research applications include assessment of hepatic enzyme induction in toxicology panels, characterization of oxidative stress biomarkers in inflammatory disease models, and quantitation of GSTa1 release during hepatocellular damage in vivo. Each kit provides reagents for 96 individual reactions in a microplate layout, enabling mid-throughput screening of treatment cohorts. Researchers aliquot reconstituted reagents to limit freeze-thaw cycles and preserve antibody binding activity over the working shelf life of the kit.