Human Glutathione Peroxidase 4 (GPX4) ELISA Kit

Glutathione peroxidase 4 is a selenocysteine-containing antioxidant enzyme that reduces phospholipid hydroperoxides in membranes and lipoproteins, acting as the master regulator of ferroptosis, an iron-dependent form of regulated cell death. The Assay Genie quantitative immunoassay measures human GPX4 protein in tissue homogenates, cell lysates, body fluids, and cell culture supernatants on pre-coated plates.

Each 96 assays kit supplies a recombinant standard, biotinylated capture antibody, biotinylated detection antibody, streptavidin-HRP conjugate, wash concentrate, substrate solution, and stop solution, with absorbance readings converted to concentration via the supplied standard curve. Reconstituted standards should be aliquoted and frozen to limit freeze-thaw cycles; the kit is intended for research use only.

Applications include ferroptosis studies in cancer cell lines, oxidative stress research in neurodegenerative disease models, and investigations of lipid peroxidation in ischemia-reperfusion injury, where GPX4 expression directly modulates cell survival outcomes.