Human Glutamate Receptor, Ionotropic, AMPA 3 (GRIA3) ELISA Kit

The Assay Genie GRIA3 immunoassay quantifies human GluA3 across a detection range of 0.312 to 20 ng/mL, with a lower limit of detection of 0.116 ng/mL, and is calibrated against recombinant GRIA3 (UniProt P42263) reference material. The procedure completes in approximately 3 hours, after which absorbance is read on a standard microplate reader. Specificity testing shows no significant cross-reactivity with homologous AMPA receptor subunits or related analogues, supporting selective measurement of GluA3 in human tissue homogenates and cell lysates. Standards are supplied lyophilized at 20, 10, 5, 2.5, 1.25, 0.625, and 0.312 ng/mL for seven-point curve generation. Unopened kits are shelf-stable for 12 months under refrigerated storage, and reconstituted reagents should be aliquoted and frozen to limit freeze-thaw cycles. Typical applications include profiling GluA3 expression in postmortem brain tissue, characterizing neuronal cell line models of AMPA receptor trafficking, and screening pharmacological compounds that modulate AMPA-type glutamate signaling in neuroscience research workflows.