Human Glutamate Cysteine Ligase, Modifier Subunit (GCLM) ELISA Kit

Quantitative measurement of the human glutamate-cysteine ligase modifier subunit (GCLM) is supported by this microplate ELISA from Assay Genie. GCLM is the regulatory modifier subunit of glutamate-cysteine ligase, the enzyme that catalyzes the first and rate-limiting step in glutathione biosynthesis, so the assay provides a protein-level readout relevant to redox homeostasis, oxidative stress, detoxification pathways, and hepatic or neuronal glutathione metabolism studies.

The kit is configured as a 96-assay microplate format with an absorbance-based readout, and is validated for human samples including tissue homogenates and cell lysates. Researchers comparing GCLM expression between treated and control groups, characterizing gene-knockout or knockdown phenotypes, or profiling glutathione-pathway changes in disease models can use the assay workflow to generate quantitative concentration data across experiments. The protocol follows a standard immunoassay procedure with a recombinant GCLM standard curve, making it suitable for batch processing of multiple samples in academic and pharmaceutical laboratories.

Each Assay Genie kit ships with antibody-coated wells, detection reagent, recombinant standards, and substrate components needed to complete the full protocol. The assay is intended for research use only and is optimized specifically for human GCLM protein detection in translational and basic research settings.