Human Glucagon-like peptide 1 receptor/GLP1R ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is designed for the quantitative measurement of Human Glucagon-like peptide 1 receptor (GLP1R) in human samples. The assay uses a double-antibody sandwich ELISA format with a colorimetric readout, providing high sensitivity and specificity for research applications studying GLP1R in the context of metabolic disorders such as diabetes and obesity.

GLP1R is described as a key receptor that mediates the effects of glucagon-like peptide-1 (GLP-1), a hormone that regulates glucose homeostasis and satiety, offering valuable insights into the receptor's role in various physiological and pathological processes.

Verified linearity in serum (90-105%, 97-101%, 90-100%), EDTA plasma (85-100%, 83-100%, 85-98%) and heparin plasma (88-101%, 84-98%, 87-93%) supports dilutions of 1:2, 1:4 and 1:8. Recovery ranges are 86-99% in serum (average 93%), 87-100% in EDTA plasma (average 93%) and 91-105% in heparin plasma (average 99%). The protocol follows a primary incubation at 37°C for 90 minutes, a 60-minute biotin-labeled detection antibody step, 30 minutes of HRP-Streptavidin binding and TMB substrate development for 10–20 minutes, with absorbance read at 450 nm. Kit components are stored at 2-8°C for 12 months.