Human GLI4 ELISA Kit (HUFI04121)

The Human GLI4 ELISA Kit from Assay Genie targets GLI family zinc finger 4 (GLI4), a zinc-finger transcription factor linked to Hedgehog signaling pathway research. The workflow is a sandwich immunoassay on a 96-well microplate pre-coated with capture antibody specific to human GLI4, with detection via a biotinylated secondary antibody and streptavidin-HRP. Validated matrices include serum and plasma, with a recommended sample volume of 50 µL per well. Investigators studying developmental gene regulation, hedgehog-pathway involvement in cancer research, or comparative transcription factor profiling can apply this ELISA to quantify GLI4 protein levels in human specimens. Reagents are intended for research use only in laboratory settings and are not approved for clinical diagnostic procedures.