Human GITR PharmaGenie ELISA Kit (SBRS0633)

Quantitative measurement of glucocorticoid-induced TNF receptor-related protein (GITR), also known as TNFRSF18 and AITR, in human research samples is supported by this immunoassay from Assay Genie. GITR is a co-stimulatory member of the TNF receptor superfamily expressed on activated CD4+ and CD8+ T cells, regulatory T cells, and NK cells, where engagement by its ligand GITRL modulates T cell proliferation, effector function, and Treg suppressive activity — making it a target of active interest in tumor immunology, checkpoint research, and autoimmune disease studies.

The kit is configured for 96 assays and includes pre-coated microplate wells, biotinylated detection antibody, streptavidin-HRP conjugate, a standardized recombinant human GITR calibrator, and a substrate solution that yields an absorbance signal proportional to bound analyte. Compatible sample matrices span cell culture supernatants, cell lysates, tissue lysates, plasma, and serum, supporting flexible study designs across in vitro cell-based experiments and ex vivo specimens.

The bench protocol runs four to five hours at room temperature, with reagents stored refrigerated between uses to preserve antibody and enzyme conjugate activity. Researchers investigating immune checkpoint combinations, Treg depletion strategies, or agonistic GITR antibody pharmacology will find this reagent aligned with cytokine profiling, T cell activation studies, and downstream western blot or flow cytometry validation work.