Human GIT1 (ARF GTPase-activating protein GIT1) ELISA KIT (HUFI05192)

The GIT1 analyte measured by this Assay Genie immunoassay is a 770-amino-acid ArfGAP scaffolding protein of approximately 85 kDa, best known for its role at focal adhesions, where it coordinates PAK and PIX signaling to regulate cytoskeletal remodeling, receptor endocytosis, and directed cell migration. Investigators studying neuronal synapse formation, integrin signaling cascades, and metastatic dissemination rely on quantitative measurement of GIT1 expression to track pathway activity across human samples.

The microplate kit ships with pre-coated capture wells, lyophilized recombinant standards, a biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer concentrate, TMB substrate, and stop solution. Unopened components are stored at 4 degrees C; reconstituted standards and working antibody aliquots should be frozen at -20 degrees C to minimize freeze-thaw loss and preserve the calibration curve across repeated runs.