Human Ghrelin PharmaGenie ELISA Kit (SBRS0025)

Quantitative measurement of human ghrelin with the Assay Genie competitive ELISA kit supports research on the 28-amino acid appetite-regulating peptide hormone. The 96-assay size provides enough throughput for cohort and time-course studies of acylated and desacyl ghrelin, two biologically distinct forms of the molecule that differ by an octanoyl modification on serine-3. The kit accepts cell culture supernatant and serum samples, accommodating both clinical metabolic studies and in vitro enteroendocrine or pituitary cell experiments. Because the competitive format tolerates the small size of the ghrelin peptide (approximately 3.3 kDa), it reports concentration reliably against a standard curve. The workflow uses pre-coated plate wells, a set of calibrators, detection antibody, and substrate, and the assay is read on a standard microplate reader, integrating easily into existing laboratory pipelines. Applications include obesity, cachexia, bariatric surgery follow-up, Prader-Willi syndrome, type 2 diabetes, and growth hormone secretion research, where circulating ghrelin dynamics, postprandial suppression, or ghrelin responses to pharmacological challenge are being characterized.