Human GFPT2 ELISA Kit (HUFI04123)

Targeting the mitochondrial paralog of the hexosamine biosynthesis pathway's rate-limiting enzyme, the Assay Genie Human GFPT2 ELISA Kit quantifies Glutamine-Fructose-6-Phosphate Transaminase 2 (GFPT2) in research specimens. GFPT2 channels glutamine and fructose-6-phosphate into UDP-GlcNAc, the donor used for dynamic O-GlcNAc modification of transcription factors, metabolic enzymes, and signaling proteins. Investigators studying insulin resistance, diabetic nephropathy, tissue fibrosis, and cancer metabolic reprogramming measure this enzyme to track pathway flux across patient-derived material. The kit ships as a complete 96 Assays colorimetric microplate-based ELISA package with pre-coated wells, biotinylated detection antibody, streptavidin-HRP conjugate, calibrated recombinant standards, wash buffer, chromogenic substrate, and stop reagent for endpoint readout. Components are provided in single-use vials sized for full-plate or partial-plate workflows, and the protocol accepts serum samples and EDTA plasma collected under standard clinical handling conditions. Researchers commonly pair GFPT2 readouts with parallel quantification of GFPT1, OGT, and OGA to profile hexosamine pathway activity across disease models, treatment arms, and longitudinal studies.