Human gephyrin ELISA Kit (HUFI04140)

Developed for neuroscience and synaptic biology research, this quantitative immunoassay from Assay Genie targets gephyrin, a 93 kDa scaffolding protein that anchors inhibitory glycine and GABA_A receptors at postsynaptic sites. Mutations in the GPHN gene have been linked to molybdenum cofactor deficiency, autism spectrum disorder, and several epilepsy syndromes, making gephyrin a high-interest biomarker across neuropsychiatric and neurodevelopmental studies. The two-site immunoassay is run on a pre-coated microplate with biotin-streptavidin-HRP signal generation, intended for research use only in vitro applications. Reconstituted standards and prepared samples are added to wells, followed by incubation with detection antibody and substrate, with absorbance measured on a compatible plate reader. Reagent components should be refrigerated on arrival and protected from light, with the lyophilized standard aliquoted and held frozen at -20 degrees C to limit freeze-thaw cycling and preserve calibration integrity. The pack contains one microplate supporting batched screening of clinical cohorts, transgenic animal model samples, or cultured neuron-derived media. Researchers investigating inhibitory synapse assembly, postsynaptic density composition, and receptor clustering mechanisms will find this kit useful for quantitative protein measurement in experimental neuroscience workflows.