Human GCNT2 ELISA Kit (HUFI07872)

From Assay Genie, this Human GCNT2 ELISA Kit quantitatively measures glucosaminyl (N-acetyl) transferase 2, the I-branching β1,6-N-acetylglucosaminyltransferase (EC 2.4.1.150) that converts linear i antigens into branched I antigens during O-glycan maturation. The assay uses a human-specific capture antibody paired with a biotinylated detection reagent and streptavidin-HRP conjugate, with no measurable cross-reactivity against the murine Gcnt2 ortholog, supporting research in transfusion medicine, glycobiology, and ophthalmology. Common applications include profiling developmental regulation of branched O-glycans in erythroid and epithelial cells, screening adult i blood group variants, and studying GCNT2 mutations associated with autosomal recessive congenital cataracts. Each kit is supplied as a complete workflow with a pre-coated microplate, calibrated protein standards, assay diluent, wash buffer, and chromogenic substrate sufficient for 96 individual determinations. Reagents are shipped refrigerated; store at 4 °C protected from light and avoid repeated freeze-thaw cycles to preserve activity through the printed expiration date.