Human GCAT ELISA Kit (HUFI04156)

The Human GCAT ELISA Kit from Assay Genie quantifies glycine C-acetyltransferase, a mitochondrial-matrix enzyme that catalyzes a key step in threonine catabolism and links to glycine metabolism. Researchers studying amino acid flux, mitochondrial enzyme expression, or metabolic pathway regulation commonly use this assay in cell biology and biochemistry workflows.

The kit ships with a precoated microplate, lyophilized standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, substrate solution, and stop reagent sufficient for a full plate of unknowns and standards. Recommended storage is 4 degrees C for most components, with the standard handled separately to limit freeze-thaw cycles, and all reagents should equilibrate to room temperature before the run.

Beyond the standard sample matrices, the assay is validated with cell lysates and tissue homogenate preparations, supporting research into glycine metabolism, mitochondrial dysfunction, and metabolic disorder models. Each plate provides quantitative absorbance-based readouts suitable for interpolation against the included standard curve, and the entire protocol can be completed within a standard laboratory workday for routine throughput.