Human GBA2 ELISA Kit (HUFI04164)

GBA2 (glucosylceramidase beta 2, non-lysosomal) is an enzyme that cleaves glucosylceramide to release glucose and ceramide at neutral pH, residing mainly in the endoplasmic reticulum and Golgi apparatus. Loss-of-function mutations in this gene are responsible for hereditary spastic paraplegia type 46 (SPG46) and cerebellar ataxia with peripheral neuropathy, while altered GBA2 expression has been observed in Parkinson's disease models and in lipid scavenging by intracellular pathogens.

This quantitative ELISA kit from Assay Genie provides reagents to measure human GBA2 protein in research specimens, supporting investigations into sphingolipid metabolism, neurodegeneration biomarker discovery, and lysosomal-storage disease pathways. Each kit includes a pre-coated microplate, biotinylated detection antibody, lyophilized recombinant standards, streptavidin-HRP conjugate, concentrated wash buffer, chromogenic substrate, and stop reagent — enough material for one full plate.

The kit ships refrigerated on gel packs; reconstituted standards should be aliquoted and frozen for long-term storage, and working solutions remain active for short-term use under refrigeration. The protocol accommodates a 100 uL sample volume per well with a standard curve run in duplicate, and researchers routinely apply the assay to cell lysates, tissue homogenates, and patient-derived specimens in vitro under research use only conditions.