Human GATA4/GATA Binding Protein 4 ELISA Kit

A high-quality Sandwich ELISA (Double Antibody) from Assay Genie designed for the quantitative measurement of GATA4 (GATA Binding Protein 4) in human samples. The assay uses a colorimetric detection method and is suitable for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with reactivity confirmed for human specimens.

Performance specifications include a sensitivity of 0.938 ng/mL and a standard range of 1.563-100 ng/mL. The kit is intended for storage at 2-8°C for 12 months and is targeted to research in epigenetics and nuclear signaling. GATA4 is described as a key transcription factor involved in cardiac development, cell differentiation, and organogenesis, with dysregulation implicated in heart defects, cancer, and diabetes.

The workflow comprises a primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody incubation at 37°C for 60 minutes, HRP-Streptavidin binding at 37°C for 30 minutes, TMB substrate development in the dark for 10-20 minutes, and absorbance reading at 450 nm after addition of stop solution. Reported dilution linearity (n = 5) covers 1:2, 1:4 and 1:8 in serum (87-98%, 83-99%, 83-99%), EDTA plasma (85-104%, 83-100%, 80-100%) and heparin plasma (85-100%, 85-101%, 84-95%). Spike-and-recovery averages are 98% for serum, 95% for EDTA plasma and 96% for heparin plasma.