Human Gastric Intrinsic Factor (GIF) ELISA Kit

Built around a competitive immunoassay format, the Assay Genie Human Gastric Intrinsic Factor (GIF) ELISA Kit supports quantitative measurement of GIF in research applications spanning gastroenterology, autoimmune disease, and nutritional science. Gastric intrinsic factor is a glycoprotein secreted by parietal cells of the gastric mucosa and serves as the carrier required for absorption of dietary vitamin B12 in the terminal ileum. Disruption of GIF production, whether through autoimmune destruction of parietal cells, atrophic gastritis, or gastric surgery, leads to cobalamin malabsorption and pernicious anemia, which is why GIF quantification remains a focus of both clinical and basic research.

Validated for human sample testing in a microplate layout, this kit accommodates multiple human specimen types and is read colorimetrically using a standard plate reader. Common research applications include profiling GIF levels in autoimmune gastritis cohorts, exploring associations with parietal cell autoantibodies, monitoring gastric function following bariatric surgery, and examining the interplay between GIF status, Helicobacter pylori infection, and cobalamin deficiency. The kit is supplied with the reagents and protocol needed to perform the full set of determinations and is intended for research use only, not for diagnostic procedures.