Human Gas 1 PharmaGenie ELISA Kit (SBRS0612)

The Growth Arrest Specific 1 (Gas1) protein is a 36 kDa GPI-anchored cell surface regulator that drives G0/G1 transition control and modulates p53-dependent tumor suppression. This Assay Genie immunoassay supports quantitative studies of proliferation arrest, apoptosis, neural development, and retinal differentiation in human research models, with a human-specific capture antibody raised against the recombinant human Gas1 sequence.

Each kit contains a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP conjugate, recombinant standards, and assay diluent. The standard curve is generated across a seven-point serial dilution, and sample replicates are recommended to improve precision. Reconstituted standards should be aliquoted and stored frozen, with repeated freeze-thaw cycles avoided to preserve immunoreactivity of the capture reagent.

Researchers commonly apply this kit to screen conditioned media from transfected cell lines, donor-matched clinical specimens, and tumor lysates when investigating growth suppression phenotypes. The protocol is compatible with standard plate readers and does not require specialized instrumentation beyond basic absorbance capability.