Human GAPT (Protein GAPT) ELISA KIT (HUFI05172)

The Assay Genie Human GAPT ELISA Kit supports quantitative measurement of Protein GAPT, a transmembrane adaptor molecule that participates in lymphocyte signal transduction. Working on a pre-coated microplate with a human-specific capture antibody, the assay pairs a biotinylated detector antibody with streptavidin-HRP to generate a colorimetric output proportional to GAPT concentration in the test specimen. A standard 100 µL sample volume is loaded per well, alongside a dilution series of the supplied calibrator used to build the standard curve. The protocol calls for an incubation of 2 hours at 37 °C, followed by substrate development with TMB and a stop reaction. Components include the pre-coated plate, detection antibody, HRP conjugate, standards, wash concentrate, dilution buffer, substrate solution, and stop reagent. Recommended for research use only in laboratory settings investigating GAPT expression within immune cell biology.