Human GAPDH PharmaGenie ELISA Kit (SBRS0611)

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase) is a 37 kDa cytosolic enzyme that catalyzes the sixth step of glycolysis, converting glyceraldehyde-3-phosphate into 1,3-bisphosphoglycerate. Beyond its metabolic role, GAPDH is one of the most widely referenced housekeeping proteins, routinely used as an internal loading control to normalize sample input across western blot and proteomics workflows. This Assay Genie PharmaGenie immunoassay employs a human-specific capture antibody pair, enabling quantitative measurement of endogenous GAPDH levels in research samples. It supports studies of glycolytic flux, mitochondrial function, apoptosis-related GAPDH translocation, and benchmarking of housekeeping gene expression. The kit is supplied as a complete reagent set including pre-coated microplate wells, calibrated protein standards, biotinylated detection antibody, and assay diluent. On arrival, store the kit refrigerated; aliquot working standards and sample diluents for freezing at -20 degrees C to limit freeze-thaw cycles. For research use only.