Human GALM ELISA Kit (HUFI07875)

The Human GALM ELISA Kit from Assay Genie quantifies galactose mutarotase (aldose 1-epimerase), a cytosolic enzyme that catalyzes the interconversion of α- and β-D-galactose anomers and supports normal galactose metabolism. Loss-of-function variants in the GALM gene have been linked to non-classical galactosemia, making reliable protein-level measurement valuable for metabolic disease research, hepatic carbohydrate handling studies, and biomarker investigations in clinical cohorts.

Researchers can apply this assay to human research samples, including cell lysates and tissue homogenates, broadening utility beyond routine clinical matrices. Each kit contains a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP conjugate, calibrated standards, wash buffer concentrate, and dilution buffers sufficient for the full plate format.

Reconstituted reagents should be aliquoted promptly to limit freeze-thaw exposure, with working streptavidin-HRP stored protected from light at 4 °C and unused calibrator frozen at -20 °C or below for long-term stability across shelf life. The procedure delivers a quantitative read-out suitable for kinetic and endpoint analysis, with sample volume requirements optimized to conserve precious research specimens. Intended for in vitro research use only, this kit supports applications in enzyme biology, pathway analysis, and translational investigation of carbohydrate metabolism disorders.