Human Galectin-3 PharmaGenie ELISA Kit (SBRS0605)

Quantitative measurement of human galectin-3 in translational research is supported by the PharmaGenie ELISA platform from Assay Genie. Galectin-3 is a ~26 kDa beta-galactoside-binding lectin secreted by activated macrophages, epithelial cells, and fibroblasts, and elevated extracellular concentrations serve as a biomarker of tissue remodeling across cardiovascular, oncology, and renal-fibrosis programs.

The kit accepts human-derived plasma or serum specimens, as well as conditioned medium from cultured cells, allowing investigators to bridge cell-based experiments with clinical sample panels on a single plate. Capture antibodies are pre-bound to wells, detection reagents are supplied in dropper bottles, and a calibrator set generates a standard curve suitable for routine absorbance-based readout on standard microplate readers.

Intended for research use only, components ship as a complete package with reagents sufficient for one full plate. Cold-chain handling preserves antibody activity across multiple freeze-thaw cycles once opened, and aliquoting the working detector solution is recommended for long-term storage.

Common applications include monitoring galectin-3 release in macrophage activation assays, quantifying biomarker shifts in cardiac-disease cohorts, and benchmarking anti-fibrotic compound effects in renal and hepatic cell models. Because LGALS3 transcription rises under TGF-beta stimulation, the kit also pairs with gene-expression readouts when researchers want to correlate secreted protein levels with transcript abundance.

Researchers comparing disease versus control cohorts, profiling drug response in fibrosis models, or screening culture supernatants after LGALS3 modulation can adopt this kit directly into existing ELISA workflows without optimizing coating conditions.