Human GAB3 ELISA Kit (HUFI04213)

This kit from Assay Genie targets GRB2-associated binding protein 3 (GAB3), a scaffolding adapter of the Gab family that modulates downstream signaling from receptor tyrosine kinases and contributes to immune cell function, including mast cell activation and macrophage polarization toward alternative phenotypes. Researchers studying chronic inflammation, hematopoietic signaling, or tumor microenvironment biology use it to quantify GAB3 levels in patient-derived serum or plasma and correlate findings with clinical observations.

Each kit supplies a pre-coated microplate, biotinylated detection antibody, lyophilized recombinant standards, streptavidin-HRP conjugate, wash buffer concentrate, substrate solution, and stop reagent, all packaged in individually labeled vials sufficient for one full plate run. The workflow involves adding diluted standards and samples to coated wells, sequential incubation with detection antibody and enzyme conjugate, thorough washing between stages, and final substrate addition for signal generation read on a standard plate reader.

Reconstituted standards in 1 mL of diluent are aliquoted into single-use tubes and frozen immediately; sealed kit components are stored refrigerated with the substrate solution protected from light to preserve activity. For research use only.