Human G-CSF/Granulocyte Colony Stimulating Factor 3 ELISA Kit

This Sandwich ELISA Kit from Assay Genie is designed for the quantitative measurement of human G-CSF (Granulocyte Colony Stimulating Factor 3) in research samples. It employs a double-antibody sandwich format with colorimetric detection, and is validated for use with human serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid sample types.

The assay has a sensitivity of 23.438 pg/mL and a measuring range of 39.063–2500 pg/mL (1 pg = 260 mIU). Reagents are intended to be stored at 2–8°C for up to 12 months. The kit's associated UniProt entry is P09919, and the analyte is also known by the synonyms G-CSF, CSF3, C17orf33, CSF3OS, Pluripoietin, Filgrastim, and Lenograstim.

Beyond the core workflow of standard addition, biotinylated detection antibody binding, HRP-streptavidin incubation, TMB colour development, and absorbance reading at 450 nm, the manufacturer reports linearity and recovery data across serum, EDTA plasma, and heparin plasma. Sample preparation guidance for serum, plasma, tissue homogenates, cell culture supernatants, and cell lysates is provided, with further support available for additional specimen types.