Human FZD3/Frizzled-3 ELISA Kit

A high-quality Sandwich ELISA kit from Assay Genie designed for the quantitative measurement of human Frizzled-3 (FZD3) levels. The kit is reactive with human samples and supports research in neuroscience, given FZD3's role as a key player in the Wnt signaling pathway where it regulates cellular processes including cell proliferation, migration, and differentiation. Dysregulation of FZD3 has been implicated in cancer, developmental disorders, and neurological conditions, underscoring its relevance as a biomarker for disease research and therapeutic investigations.

The assay uses a sandwich ELISA format with a double-antibody configuration and colorimetric detection. Sensitivity is reported at 0.469 ng/mL with a detection range of 0.781-50 ng/mL. Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Reported linearity for serum (n=5) is 85-98% at 1:2, 85-101% at 1:4, and 89-99% at 1:8 dilution. EDTA plasma (n=5) shows 84-100% at 1:2, 82-95% at 1:4, and 85-99% at 1:8, while heparin plasma (n=5) shows 81-97% at 1:2, 83-100% at 1:4, and 83-93% at 1:8. Recovery is 85-103% (average 97%) for serum, 86-98% (average 90%) for EDTA plasma, and 90-104% (average 98%) for heparin plasma.

Storage is at 2-8°C for 12 months. Associated synonyms for the target include FZD3, Frizzled-3, frizzled Drosophila homolog 3, frizzled homolog 3 (Drosophila), frizzled-3, Fz-3, and hFz3, with the UniProt reference Q9NPG1.