Human FUT9 ELISA Kit (HUFI04218)

The Assay Genie Human FUT9 ELISA Kit enables quantitative measurement of Fucosyltransferase 9, a Golgi-resident glycosyltransferase that catalyzes the transfer of fucose from GDP-fucose to N-acetylglucosamine residues, generating Lewis x and Lewis y carbohydrate epitopes on glycoproteins and glycolipids. Researchers studying glycobiology, tumor-associated antigen expression, and innate immune cell signaling rely on this microplate-based assay to track FUT9 protein across human research samples.

The antibody pair is specific for human FUT9, with no significant cross-reactivity to rat or mouse fucosyltransferase orthologs. Each kit includes a pre-coated microplate with removable strip wells, biotinylated capture antibody, streptavidin-HRP conjugate, lyophilized recombinant standards, concentrated wash buffer, TMB substrate, and stop solution supplied in individual vials. A 100 µL aliquot of wash buffer is typically applied per well during each washing step.

Reconstituted reagents should be refrigerated upon receipt and stored frozen for long-term preservation; protect unopened components from light and limit freeze-thaw cycles to maintain antibody binding activity. The assay is validated for in vitro research use only and supports investigations into glycosyltransferase regulation in colorectal, breast, and lung carcinomas, where altered fucosylation patterns influence cell adhesion, stem-cell differentiation, and inflammatory responses involving selectin-ligand biosynthesis.