Human FUT4 ELISA Kit (HUFI04215)

Assay Genie's ELISA platform provides quantitative measurement of human fucosyltransferase 4, a Golgi-resident glycosyltransferase that catalyzes alpha-1,3-fucosylation reactions essential for Lewis X (CD15) antigen biosynthesis and selectin-ligand assembly on leukocyte and tumor-cell surfaces. Investigators in oncology, hematology, and inflammation research apply the assay to monitor enzyme-level changes across cultured cell lines, primary tissue lysates, and processed blood-derived specimen fractions.

The kit is supplied as a complete 96 Assays configuration with pre-coated microplate strips, biotinylated detection antibody, streptavidin-HRP conjugate, recombinant calibrator protein, and calibrated standard dilutions. A standard curve is generated from a serial dilution of recombinant human FUT4 reference material, allowing interpolation of unknown analyte concentrations against the included internal calibrator. The protocol follows a four-step binding sequence with intervening wash stages, and TMB substrate turnover is terminated with an acid stop solution before endpoint absorbance recording on a standard microplate reader.

Intended for research use only, the workflow supports biomarker discovery in cancer progression, immune-cell differentiation, and glycosylation-engineering studies. Reagents should be stored refrigerated at 4 degrees C upon receipt; component integrity is maintained when the kit remains sealed and protected from light between uses. Each kit provides materials for a single plate run, with working aliquots intended to limit repeated freeze-thaw cycles on the detection reagents.

Assay Genie ships the product with lot-specific validation documentation and a step-by-step protocol booklet, supporting laboratory personnel who routinely perform manual wash protocols, standard curve generation, and curve-fitting analysis.