Human FUBP1 ELISA Kit (HUFI04224)

FUBP1 (Far Upstream Element Binding Protein 1) is a single-stranded DNA-binding transcription factor that binds the far upstream element of the MYC promoter and influences expression of cell-cycle and proliferation genes. This quantitative immunoassay kit from Assay Genie is built for researchers investigating FUBP1 regulation, MYC pathway biology, and biomarker discovery in tumor and neurological studies.

The microplate-based workflow includes pre-coated wells, matched capture and detection antibodies, recombinant protein standards for curve generation, sample and wash buffers, and substrate solution. Researchers prepare diluted clarified specimens and load them onto the plate well by well, with absorbance then read on a standard plate reader. Curve fitting against the included standards allows interpolation of unknown concentrations.

FUBP1 has been linked to oligodendroglioma, hepatocellular carcinoma, and polycythemia vera through published mutational and expression studies, making this kit useful for in vitro experiments profiling tumor cell lines, primary tissue lysates, and conditioned media. The kit ships with a lot-specific certificate of analysis and a printed protocol booklet; unopened vials and reagents are stored refrigerated and protected from light until first use.