Human FTO (Alpha-ketoglutaRate-dependent dioxygenase FTO) ELISA Kit (HUFI05245)

The Assay Genie ELISA kit for human FTO enables quantitative measurement of the alpha-ketoglutarate-dependent dioxygenase FTO in research samples, supporting studies into m6A RNA demethylation, obesity-related signaling, and metabolic disease pathways. FTO is a nuclear Fe(II)/2-oxoglutarate-dependent demethylase that removes methyl groups from N6-methyladenosine residues on nuclear RNA, and altered expression has been linked to adipogenesis, energy homeostasis, type 2 diabetes, and several malignancies including acute myeloid leukemia and glioblastoma. The kit is configured for 96 Assays per box and uses a colorimetric readout compatible with standard microplate readers, making it practical for screening panels of human-derived specimens in academic and translational research settings. Each kit package includes a pre-coated microplate and the reagents required to generate a standard curve, with the assay intended for in vitro research use only and not for diagnostic applications. Storage should follow the cold-chain conditions provided with the kit, and laboratories should consult the enclosed protocol for sample preparation, recommended dilution factors, and plate-reading parameters specific to this product.